March 2011 - MARMAM - devlists.uvic.ca

PAM operator vacancy, Ocean Science Consulting Ltd, Scotland, UK
by Rebecca Thompson 23 Mar '11

23 Mar '11

Dear all, OSC has vacancies for the upcoming summer season for EXPERIENCED passive acoustic monitoring (PAM) operators. The PAM operator must have the minimum of 12 weeks experience operating a PAM system, six weeks of which must have been a lead position (stated on CV). They must have the ability to install a basic operating system and PAM software (e.g. PAMGUARD and IFAW) software onto new laptops, set up National Instrument sound cards, and configure PAM with a hydrophone array and portable GPS unit or with vessel's NMEA (satellite) comms. They must also be competent deploying and recovering the hydrophone array in a range of situations, and they must be skilled in the acoustic identification of marine mammals. The PAM operator will also be required to trouble shoot software and hardware issues independently. Previous OSC applicants need not apply and candidates without the experience mentioned above need not apply. Applicants should possess the minimum of an undergraduate science-based degree (including engineering) with a minimum grade of upper second class honours (2.1), valid offshore survival training certificates and associated medicals. Preference will be given to candidates that also hold valid certificates for work in Norwegian and Dutch waters. Early applicants may be considered for a job commencing 15^April 2011. To apply, please e-mail your CV (Word 2003 or earlier) and associated certificates in JPEG format to Rebecca Thompson, Operations Co-ordinator at rt(a)osc.co.uk. Regards Rebecca Thompson -- Rebecca Thompson Operations Co-ordinator OSC Limited Ocean House 4 Brewery Lane Belhaven, Dunbar East Lothian, Scotland EH42 1PD M: +44 (0)7706 662 339 T: +44 (0)1368 865 722 F: +44 (0)1368 865 729 W: www.osc.co.uk

1 0

New paper on cetacean DNA barcoding
by AMELIA VIRICEL 22 Mar '11

22 Mar '11

Dear Marmam Readers: We are pleased to announce the publication of the following paper in Marine Mammal Science: Evaluating the utility of cox1 for cetacean species identification A. Viricel and P.E. Rosel Article first published online: 17 MAR 2011 DOI: 10.1111/j.1748-7692.2010.00460.x If you are interested in a pdf please email request to: viricel[at]louisiana[dot]edu For subscribers to Marine Mammal Science, it can be downloaded from: http://onlinelibrary.wiley.com/doi/10.1111/j.1748-7692.2010.00460.x/abstract Abstract: The mitochondrial cytochrome c oxidase I (cox1) gene has been promoted as a universal reference gene, or barcode, to identify organisms to the species level. We evaluated whether cox1 would be appropriate to diagnose cetacean species. The 5′ end of cox1 (686 base pairs, bp) was sequenced for 46 of 86 recognized species of cetaceans. In addition, we included 105 sequences from GenBank, increasing our taxonomic coverage to 61 species. Particular focus was placed on sampling two subfamilies that contain closely related taxa: the Delphininae and the Globicephalinae. Species-specific sequences were observed for all but three taxa (Delphinus delphis, D. capensis, and Stenella coeruleoalba). Although correct assignment was seen for most species, significant overlap between intra- and interspecific variation makes cox1 an imperfect barcode for cetaceans. The efficacy of cox1 was compared to the 5′ end of the cytochrome b (cytb) gene, a mitochondrial region routinely used for cetacean species identification. Although cytb performed better than cox1 for some species, this marker could not differentiate other closely related taxa (Eubalaena spp.). Species identification for taxa not reliably identified using cox1 or cytb might be best addressed through use of multiple mitochondrial DNA fragments or other newly developed markers. Best regards, Amélia Viricel Amélia Viricel, Ph.D. Candidate University of Louisiana at Lafayette, Biology Dept. 300 E St Mary Blvd, Billeaud Hall rm 108 Lafayette LA 70504 USA

1 0

Job Posting: Postdoc in bioacoustic signal detection, Cornell University
by Aaron N. Rice 22 Mar '11

22 Mar '11

*Postdoctoral Associate- Animal Acoustic Signal Detection, Classification and Localization *(position 14540) *Bioacoustics Research Program, Cornell Laboratory of Ornithology, Cornell University* The Bioacoustics Research Program (BRP) is a diverse group of scientists and engineers focused on studying the natural sound-scape and animal vocal signals. This position will join BRP's Application Science Engineering (ASE) team to focus on the development and implementation of automatic detection, classification, and localization algorithms specifically related to processing large quantities of bioacoustic data. * Collaborate with researchers and software programmers on developing systems and methods that facilitate the rapid processing of acoustic data, writing and publishing manuscripts, and assisting with grant applications. * Collaborate with researchers in multiple programs in the Lab as well as other algorithm developers. * Survey available research and incorporate methods and algorithms. * Conduct original scientific research in the field of bioengineering, animal communication, and bioacoustics. * Perform research and development of novel signal processing and machine learning algorithms which can be applied to the detection, measurement, classification, and quantitative comparison of animal sounds. * Conceive of, develop, and implement complex code providing robust solutions to specific and general research needs. * Provide leadership in core research areas by providing training and technical presentations for internal and external audiences. * Participate in the preparation and implementation of project plans. * Provide input into project management systems including effort allocation for self and co-workers. * Obtain an understanding of process engineering and utilize these skills to provide organized methods and practices to meet project deadlines and technical goals. /Qualifications/ * PhD in engineering or related field, with particular focus in signal processing. * Demonstrated experience in algorithm development. * Strong programming ability. * Experience with acoustics and/or marine mammal research preferred. * Experience in embedded programming a plus. * Ability to work effectively both in a team and independently. * Experience in scientific programming and the generation of highly complex computer code in MATLAB and other high-level languages such as C/C++. * Ability to conduct original research, from conception to completion, and to develop innovative technical solutions to support that research. * Experience in literature review and publishing scientific papers. * Must have strong communication skills, efficient scientific writing capability, and be proficient in spoken and written English. To apply, send a letter of application, complete résumé or CV, list of references and academic transcripts to: Susan Taggart, Cornell Lab of Ornithology, 159 Sapsuckers Woods Road, 283A Johnson Center for Birds & Biodiversity, Ithaca, NY 14850 or by email to set2(a)cornell.edu <mailto:set2@cornell.edu>. Inquiries about position specifics can be directed to Peter Dugan at 607-254-1149, pjd78(a)cornell.edu <mailto:pjd78@cornell.edu> or Harold Cheyne at 607-254-6280, hac68(a)cornell.edu <mailto:hac68@cornell.edu>. Review of applicants will begin immediately and continue until the position is filled. Located at the Imogene Powers Johnson Center for Birds and Biodiversity in the 220-acre Sapsucker Woods sanctuary, the Cornell Lab of Ornithology is the world's leading resource for conservation, research, education, and citizen science focused on birds. The lab is a vibrant unit within Cornell's College of Agriculture & Life Sciences, where several full-time faculty teach undergraduate courses, advise graduate students, and manage world-class, mission-driven programs. Our management and staff are committed to the highest standards of ethics and excellence in all areas of our work, and our Board leadership includes faculty from Cornell and other universities, successful entrepreneurs and managers from the business and non-profit sectors, and conservation-minded citizens from the United States and beyond. Cornell University, located in Ithaca, New York, is an inclusive, dynamic, and innovative Ivy League university and New York's land-grant institution. Its staff, faculty, and students impart an uncommon sense of larger purpose and contribute creative ideas and best practices to further the university's mission of teaching, research, and outreach. /Cornell University is an equal opportunity, affirmative action educator and employer./

1 0

NWSSMM 15th meeting
by Frances Robertson 22 Mar '11

22 Mar '11

15th Northwest Student Chapter Meeting for the Society of Marine Mammalogy Register for the meeting at http://www.surveymonkey.com/s/SNKSQMG Our apologies for cross-posting. This year's meeting is to be held at the University of British Columbia on the 7 May 2011. Meeting registration and abstract submission is now open. The deadline for registration is the 15 April. We welcome all those interested to attend, student or not. Highlights of the meeting: * Student presentations * Student poster session * Plenary presentations by Dr. Dr. John Calambokidis of Cascadia Research and by Dr. Dom Tollit of SMRU Ltd. * Tour of the blue whale skeleton at the Beaty Biodiversity Museum (optional) * Saturday night potluck dinner (optional) * Sunday morning tour (optional) Registration and abstract submission: Registration for the 2011 NW Student Chapter meeting for the Society of Marine Mammalogy is now open. The deadline for registration will be the 15 April. This year we have produced an online form in order to make the process of registration quicker and easier for you. Please ensure you click the "done" button when you have completed the survey or it will not submit your response. Please follow the link below for registration. http://www.surveymonkey.com/s/SNKSQMG The registration fee for the meeting will be finalized after all registrations have been received, but in the past has ranged between $15 and $20. This registration fee includes breakfast, lunch, and snacks, but does not cover additional costs for the Saturday dinner, Sunday event, or accommodations in Vancouver. We do have limited accommodations available with students, and we will be able to offer these preferentially to those who are presenting or participating in the optional social events. While we encourage everyone working with or interested in marine mammal research to attend, only students are eligible to present at the meeting. All students are encouraged to present their work, whether this be a completed project, an update, a side-project, or a proposal. Due to the growing numbers of attendees over recent years we are trying something new this year. We would like to include a poster session in order to provide an opportunity to everyone that wishes to share their work at the meeting. If you are interested in presenting, please include the title of your presentation and an abstract (approximately 250 words) on your registration form, and indicate whether you would prefer an oral or poster presentation. We will do our best to accommodate everyone's preferences. If you are interested in a list of presentations given at previous chapter meetings, go to this link <http://www.marinemammalscience.org/index.php?option=com_content&view=ar ticle&id=136&Itemid=171> . Social Events: We are hosting three optional events following the student chapter meeting. Immediately following the chapter meeting, Dr. Andrew Trites will be giving a tour of the Blue Whale skeleton exhibited in the Beaty Biodiversity Museum (http://www.beatymuseum.ubc.ca/) There will be no fee for this event. On Saturday evening, we will be hosting a potluck dinner. Food will be provided by the students of the Marine Mammal Research Unit. We just ask that attending guests provide beverages. On Sunday morning, we will be hosting a tour of either the UBC/Vancouver Aquarium Open Water Steller sea lion research facility (http://www.vanaqua.org/conservationinaction/sealions/open_water.htm) or a behind-the-scenes tour of the Steller sea lion and Northern fur seal research facility at the Vancouver Aquarium (http://www.vanaqua.org/) Event details will be confirmed prior to the meeting and will depend on the number of registrants. There will be no fee for this event, however, there may be a small cost associated with transportation and parking. We look forward to seeing you at this year's meeting and hearing of your work. Further details will be sent out closer to the meeting. Should you have any questions regarding the registration and/or abstract submission, feel free to email f.robertson(a)fisheries.ubc.ca or m.wong(a)fisheries.ubc.ca Cheers, Frances and Mandy Northwest Student Chapter of the Society for Marine Mammalogy <http://www.marinemammalscience.org/index.php?option=com_content&view=ar ticle&id=127&Itemid=163> Frances C. Robertson O PhD Candidate Marine Mammal Research Unit University of British Columbia AERL 2202 Main Mall Vancouver, BC Canada, V6T 1Z4 f.robertson(a)fisheries.ubc.ca http://distantfin.com CA 604 339 4967 USA 360 420 4403

1 0

Job Announcement
by Daniel Barth 21 Mar '11

21 Mar '11

The Alaska SeaLife Center, a non-profit organization generating and sharing scientific knowledge that promotes understanding and stewardship of Alaska’s marine ecosystems is seeking applications for the following: *Animal Care Technician* Under the direct supervision of the Stranding Coordinator and the Mammal Curator, this position provides for the daily and routine cleaning and maintenance of stranded mammal and avian enclosures and work areas, animal diet preparation, and record keeping. Provide support to stranding department in feeding, handling of marine mammals and marine birds within the care of the ASLC Stranding Department. This position will provide logistical support involved for animal stranding, rescue and release. * * *Responsibilities (include but are not limited to):* 1. Assists in the care and maintenance of animals managed by the Center’s Stranding Department. 1. Daily animal diet preparations, water quality testing, and routine cleaning of animal habitats, holding pools, food preparation facilities and marine mammal and avian work areas. 1. Assist with logistics pertaining live and dead strandings including rescues, animal transport and releases. 1. Maintain written and computer based records. 1. Follow procedures for animal care, food preparation, and clean up, including proper maintenance and sterilization of equipment. 1. Under direction of the Stranding Coordinator, assist research staff in facilitation of research projects and the collection of data samples, etc. 1. Participate in educational presentations to visitors and the public on topics relating to marine mammal stranding and ASLC operations. Provide helpful, patient assistance and information to the public. *Requirements*: Bachelor's degree in Marine Biology, Biology, Zoology or related field is preferred. Some captive or stranding marine mammal and/or avian experience is required. Soft and technical skills should include: exceptional time management /organizational skills; self-motivated; ability to communicate effectively with various levels in the organization; computer skills including MS Outlook, Excel, Word, and database programs; excellent written and oral communication skills. Must be physically fit with a good sense of balance and be able to lift over 50 lbs. Must have a valid driver's license. This is a seasonal, full-time position. The Alaska SeaLife Center offers a competitive wage in a beautiful setting. *Start Date: May 1, 2011 or June 15, 2011* *End Date: Approximately October 1, 2011 or November 15, 2011 * *Apply By: ** Open Until Filled * * * Send resume & application (downloadable on website) to ASLC, Human Resources, P.O. Box 1329, Seward, AK 99664, fax 907-224-6320, or email HR(a)alaskasealife.org. Affirmative Action: All qualified applicants will be considered. Minority, women, disabled veterans, campaign badge veterans, recently separated veterans or disabled are encouraged to apply. www.alaskasealife.org *The ASLC is an Equal Opportunity**/Affirmative Action Employer.*

1 0

Listen to the deep
by MARIA MORELL 20 Mar '11

20 Mar '11

Dear colleagues, This message is for your information. The Laboratory of Applied Bioacoustics (LAB) of the Technical University of Catalonia (UPC) is coordinating an international project with different institutions to monitor noise and its effects on the marine environment. Amongst these institutions are the Japan Agency for Marine-Earth Science and Tecnology (JAMSTEC) who is responsible of detecting and analysing geohazards in Japan, and the Japan National Research Institute of Fisheries (FRA). Through this collaboration, we are constantly monitoring underwater noise off Japan and could detect and record the 11 March earthquake. If you are interested, you can access this recording and live monitor the sound of the afterschocks at http://listentothedeep.com , in the "Listen on site" section. This data constitute a unique opportunity to understand the acoustic relationship between natural underwater events and the behaviour of cetaceans. With kind regards, Michel André Maria Morell Laboratori d'Aplicacions Bioacústiques Centre Tecnològic de Vilanova i la Geltrú Universitat Politècnica de Catalunya Avda Rambla Exposició s/n 08800- Vilanova i la Geltrú (Barcelona)SPAIN telf: 0034 938967290 http://www.lab.upc.es maria.morell(a)lab.upc.edu

1 0

New paper on Mediterranean sperm whales
by Alexandros Frantzis 19 Mar '11

19 Mar '11

The following paper became available on-line in /Deep Sea Research I/: Inter-basin movements of Mediterranean sperm whales provide insight into their population structure and conservation *A. Frantzis, S. Airoldi, G. Notarbartolo-di-Sciara, C. Johnson and S. Mazzariol* /Deep-Sea Research I /58 (2011) 454--459. doi:10.1016/j.dsr.2011.02.005 *Abstract: *The sperm whale is one of the very few deep diving mammal species in the Mediterranean Sea. Following a rare mass stranding of male sperm whales in the Adriatic Sea in December 2009, photoidentification methods were used in order to investigate previous sightings of the stranded whales in the region. Fluke photos of the stranded whales were compared with those of 153 and 128 free-ranging individuals photographed in the western and eastern Mediterranean basins, respectively. Three out of the seven stranded whales had been previously photo-identified and some of them more than once. To reach the stranding place, two of these re-identified whales performed long-range inter-basin movements of about 1600--2100 km (in a straight line) either through the Strait of Sicily or the Strait of Messina. In addition, comparisons among all whales photographed in the two Mediterranean basins revealed that one more individual first photographed in the western basin (1991) was re-identified 13 years later in the eastern basin (2004). These three cases provide the first conclusive evidence of interbasin movement of sperm whales in the Mediterranean Sea. Inter-basin gene flow is important for the survival of the small and endangered Mediterranean sperm whale population. Mitigating the disturbance created by human activities in the straits area is crucial for its conservation. © 2011 Elsevier Ltd. All rights reserved. For those having access to Deep Sea Research I the link for full paper download is: http://dx.doi.org/10.1016/j.dsr.2011.02.005 Otherwise please contact me for a copy at:afrantzis@otenet.gr <mailto:ana.pinela@gmail.com> Best wishes (and hope to see some of you in Cadiz), Alexandros <mailto:anapinela@ub.edu> -- ___________________________________________ Dr. Alexandros Frantzis Scientific director Pelagos Cetacean Research Institute Terpsichoris 21 16671 Vouliagmeni, GREECE Tel.: +30-210-8960108 e-mail: afrantzis(a)otenet.gr website: http://www.pelagosinstitute.gr ___________________________________________

1 0

VOLUNTEER INDO-PACIFIC BOTTLENOSE DOLPHIN RESEARCH ASSISTANTS needed from mid-April 2011
by Delphine CHABANNE 19 Mar '11

19 Mar '11

VOLUNTEER INDO-PACIFIC BOTTLENOSE DOLPHIN RESEARCH ASSISTANTS needed from mid-April 2011 The Indo-Pacific bottlenose dolphin population (Photo-Identification) research program is accessible to you even if you have never been on a boat before; have never participated in a research program; if you are alone or with friends; whatever your situation, we can provide you an experience you will likely never forget! Contributing to dolphin’s research and conservation. Where: Murdoch University, Perth, Western Australia When: From mid-April 2011 Duties: - Assisting with boat-based photo-identification surveys of bottlenose dolphins, including operating small vessel during surveys, collecting environmental, location, and behavioural data for dolphin groups - Photo-identification of dolphins in the lab. Qualifications: - Enthusiasm for field work on a boat is essential - Positions are available from mid-April 2011 with a minimum 12 weeks commitment. People able to commit for longer periods will be preferred. Preferred, but not mandatory: - Undergraduate degree in the biological sciences - Field research/small boat operation experience preferred but not required. If interested: Please submit resume and cover letter specifying how long approximately you would like to do it via email: delphine.chabanne(a)gmail.com Delphine CHABANNE Research Associate, Murdoch University Cetacean Research Unit (MUCRU) Centre for Fish and Fisheries Research Faculty of Sustainability, Environmental and Life Sciences Murdoch University Murdoch, WA 6150, Western Australia Website: http://www.cffr.murdoch.edu.au/mucru/delphine_chabanne.html Email: delphine.chabanne(a)gmail.com

1 0

PhD opportunities
by Vic Cockcroft - CDS 17 Mar '11

17 Mar '11

Hi Everyone The Centre for Dolphin Studies and the Mauritian Marine Conservation Society have an ongoing programme studying cetacean presence and biology off Mauritius. We have opportunities for two students to undertake PhD level (MSc level will be considered) studies in Mauritius. We have enough funding to undertake the research and provide a small stipend to students, enough to live on, as accommodation is provided. We have a number of potential projects, but are open also to suggestions and individual preferences. Despite our funding, we encourage applications from students currently attached to Universities and whom might have access to other funding. But, we'll consider all applications and PhD registration is available through a South African or Mauritian University. If you're interested, please contact me. Vic Cockcroft Centre for Dolphin Studies An Independent Research and Educational Institute Phone 27 (0)44 532 7954 Fax 27 (0) 865158319 Mobile/Cell: 27 (0)83 655 6902 www.dolphinstudies.org SKYPE: C.D.S.1

1 0

New publications on the ecological physiology extreme fasting in subantarctic fur seal pups
by Delphine verrier 17 Mar '11

17 Mar '11

Dear colleagues, On behalf of my co-authors, I am pleased to announce the publication of the following papers on subantarctic fur seal fasting capabilities and their ecological implications. If you are interested in PDF copies, please email me at: ddlafouine(a)free.fr Best wishes, Delphine PAPER #1: Verrier, D., Groscolas, R., Guinet, C. and Arnould, J. P. Y. , Development of fasting abilities in subantarctic fur seal pups: balancing the demands of growth under extreme nutritional restrictions. Functional Ecology, no. doi: 10.1111/j.1365-2435.2010.01823.x Summary: 1. Surviving prolonged food deprivation requires various metabolic adaptations such as energy and protein sparing, which can be highly conflicting with energy-demanding stages of an animal’s life history such as growth. 2.Due to the maternal attendance pattern, subantarctic fur seal (Arctocephalus tropicalis Gray) pups must repeatedly endure exceptionally long fasts of increasing duration throughout the 10-month lactation period. Little is known of (i) how these infants adapt to such extreme energetic constraints while sustaining growth and development; and (ii) the ecological implications of repeated prolonged fasting in early life in terms of offspring survival, maternal care and growth strategy in this species, as well as the evolutionary consequences of such life history trait. 3. Physiological responses to prolonged fasting and how they change with development throughout the pre-weaning period were investigated. Results show that beginning with their first fast, subantarctic fur seal pups are able to mobilize lipid reserves preferentially while conserving protein stores in response to nutritional deprivation. As pup age, profound changes in energy expenditure allow the implementation of an efficient strategy of fat storage and lean body mass preservation, which proves highly adaptive in the face of the low maternal provisioning rates experienced. 4. Despite increasing fasting durations, pup mortality decreased markedly throughout the maternal dependence period. Consistent with predictions, field measurements indicate that fasting endurance, although limited in early life, increases up to durations of nearly 3 months with age. Results suggest that the maternal provisioning strategy could be constrained by these ontogenetic changes in pup fasting abilities. 5. Furthermore, extreme energetic constraints and local density-dependent effects appear to exert a strong selective pressure upon the adoption of a convergent growth strategy between the sexes aiming to maximize fat storage and pre-weaning survival. 6. The issues of resulting trade-offs between pre- and post-weaning survival and the evolutionary consequences of extreme fasting abilities are also addressed. PAPER #2: Verrier, D., Guinet, C., Authier, M., Tremblay, Y., Schaffer, S., Costa, D. P., Groscolas, R. and Arnould, J. P. , The ontogeny of diving abilities in subantarctic fur seal pups: developmental trade-off in response to extreme fasting?. Functional Ecology, no.doi: 10.1111/j.1365-2435.2011.01846.x Summary: 1. A major hypothesis of life-history theory is that conditions of early development affect future survival and reproductive success. Responses to detrimental environments during early ontogeny may involve trade-offs between current and future fitness. Yet, the functional mechanisms involved in such evolutionary trade-offs remain poorly documented. 2. The physiological and behavioural ontogeny of diving abilities was examined in subantarctic fur seal (Arctocephalus tropicalis Gray) pups to assess whether the repeated extreme fasts they naturally endure throughout the period of maternal dependence impacts on their development. 3. The ontogeny of pup body oxygen storage capacity was slow, in particular for the muscle compartment, which shows limited increase in myoglobin content (0·23–0·85 g 100 g wet muscle−1). As a consequence, by the time of weaning, mass-specific oxygen stores had only reached 76%, 24% and 61% of adult female capacity for blood, muscle and total, respectively. Concomitantly, in marked contrast to other pinniped infants, they spent decreasing amounts of time in water (16–7%) with age and exhibited very little diving experience and skills. 4. Overall, in addition to experiencing the longest fasting durations throughout the maternal dependence period, subantarctic fur seal pups demonstrate the lowest levels of mass-specific total oxygen stores and maximum dive duration of any otariid near the age of weaning reported to date. 5. Furthermore, dives that exceeded the calculated aerobic dive limit occurred with a very low frequency (0·04%), suggesting that behavioural limitations linked to the necessity to conserve energy to survive repeated fasting, rather than restricted oxygen storage capacity, constrained pup diving behaviour. 6. Hence, these animals appear to trade-off the early development of both their physiological and behavioural diving abilities in favour of body fat accumulation to survive the prolonged fasts they must endure and, potentially, provide a nutritional buffer while they locate appropriate food patches after weaning. While promoting pre-weaning survival, this strategy renders pups more vulnerable to unpredictable changes in environmental conditions and food availability at the transition to independent foraging and, thus, could have negative impact on post-weaning survival. ********************************************************************************* Dr Delphine Verrier DVM, PhD Head of Primate Centre Projet leader for the "Primatology and Health" programme (URES) International Centre for Medical Research of Franceville (CIRMF) B.P. 769, Franceville, Gabon Phone : +241 67 70 62 / Mobile: +241 07 98 56 69 Email: <mailto:ddlafouine@free.fr> ddlafouine(a)free.fr Skype: ddlafouine1 ********************************************************************************* cid:34994B7F-E15C-441B-8A1E-AFDCC209897F

1 0