Dear Colleagues, My co-authors and I are pleased to announce our recent publication. Probert R, Bastian A, Elwen SH, James BS, Gridley T (2021) Vocal correlates of arousal in bottlenose dolphins (*Tursiops* spp.) in human care. PLoS ONE 16(9): e0250913. https://doi.org/10.1371/journal.pone.0250913 Abstract: Human-controlled regimes can entrain behavioural responses and may impact animal welfare. Therefore, understanding the influence of schedules on animal behaviour can be a valuable tool to improve welfare, however information on behaviour overnight and in the absence of husbandry staff remains rare. Bottlenose dolphins (*Tursiops* spp.) are highly social marine mammals and the most common cetacean found in captivity. They communicate using frequency modulated signature whistles, a whistle type that is individually distinctive and used as a contact call. We investigated the vocalisations of ten dolphins housed in three social groups at uShaka Sea World dolphinarium to determine how patterns in acoustic behaviour link to dolphinarium routines. Investigation focused on overnight behaviour, housing decisions, weekly patterns, and transitional periods between the presence and absence of husbandry staff. Recordings were made from 17h00 – 07h00 over 24 nights, spanning May to August 2018. Whistle (including signature whistle) presence and production rate decreased soon after husbandry staff left the facility, was low over night, and increased upon staff arrival. Results indicated elevated arousal states particularly associated with the morning feeding regime. Housing in the pool configuration that allowed observation of staff activities from all social groups was characterised by an increase in whistle presence and rates. Heightened arousal associated with staff presence was reflected in the structural characteristics of signature whistles, particularly maximum frequency, frequency range and number of whistle loops. We identified individual differences in both production rate and the structural modification of signature whistles under different contexts. Overall, these results revealed a link between scheduled activity and associated behavioural responses, which can be used as a baseline for future welfare monitoring where changes from normal behaviour may reflect shifts in welfare state. Please feel free to contact me if you have any questions: rachelprbrt@gmail.com Best regards, -- Rachel Probert PhD candidate, Marine Biology, Bioacoustics Sea Search Research and Conservation, Muizenberg, Cape Town School of Life Sciences, University of KwaZulu-Natal, Westville Cell: +27767831809 https://www.buymeacoffee.com/SeaSearch