Dear MARMAM community, On behalf of my coauthors, I am pleased to share our recently published article in Aquatic Conservation: Marine and Freshwater Ecosystems. Carvalho, C.C., Marmontel, M., Botta, S., Emin-Lima, R., Costa, A.F., Crema, L.C. et al. (2022). Spatio-temporal, ontogenetic and sex-related patterns in resource use of Amazonian manatees across floodplains and estuaries as inferred by δ13C and δ15N isotopic values. Aquatic Conservation: Marine and Freshwater Ecosystems, 1– 14. https://doi.org/10.1002/aqc.3833 Abstract Threats to aquatic mammals may occur in isolation or in combination, leading to cumulative effects on populations and their ecosystems. Thus, conservation actions must consider the complexity of factors involved in patterns of habitat use, including feeding ecology, such as ontogenetic, spatial and temporal dietary shifts. The Amazonian manatee (Trichechus inunguis) occurs in a variety of environments, such as várzeas and igapós (flooded areas) and estuaries. Different environments can lead to variation in the diet of manatees according to the availability and abundance of plant species. Stable isotope analysis (δ13C and δ15N) of manatee teeth was performed to assess spatio-temporal, ontogenetic and sex-related patterns in resource use. Mixing models were applied to identify the most consumed plant groups. In várzeas, there were significant interactions between sex and ontogenetic class and ontogenetic class and time series for δ13C values. For manatees sampled from 1994 to 2004, males and adults showed the highest δ15N values, and aquatic C3, followed by C4 plants, were the groups that most contributed to the species’ diet. Comparisons among the environments showed the highest δ13C values for manatees from várzeas and the lowest values for manatees from igapós. Várzeas had the highest abundance of C4 plants (high δ13C values), whereas they are scarce in igapós, leading manatees to consume mainly C3 species. The isotopic values of manatees mirrored those of the most available food sources in each environment, supporting the generalist and opportunist behaviour described for the species. Therefore, threats to the diversity, distribution and abundance of aquatic macrophytes, such as contamination, cattle raising and changes in the hydrological cycle (as a consequence of dams and climate change), will affect the diet of the manatee and should be taken into account when designing conservation strategies for the species. You can also contact me directly for a PDF copy: camilacarvalho.bio08@gmail.com Kind regards, Camila Carvalho -- Camila Carvalho de Carvalho Especialização em Jornalismo Científico - LabJor/Unicamp Mestre em Oceanografia Biológica - PPGOB/FURG Grupo de Pesquisa em Mamíferos Aquáticos Amazônicos - IDSM skype: camila.carvalho.de.carvalho cel: +55 15 98151-9531