New publication about lacaziosis-like disease in Tursiops truncatus from Brazil
Dear Colleagues: I am pleased to announce the publication of our research article about Lacaziosis-like disease: Sacristán C, Réssio RA, Castilho P, Fernandes NCCA, Costa-Silva S, Esperón F, Daura-Jorge FG, Groch KR, Kolesnikovas CKM, Marigo J, Ott PH, Oliveira LR, Sánchez-Sarmiento AM, Simões-Lopes PC, Catão-Dias JL (2015). *Lacaziosis-like disease in Tursiops truncatus from Brazil: a histopathological and immunohistochemical approach*. Dis Aquat Org 117:229-235 ABSTRACT: Cetacean lacaziosis-like disease or lobomycosis-like disease (LLD) is a chronic skin condition caused by a non-cultivable yeast of the order Onygenales, which also includes *Lacazia loboi*, as well as *Paracoccidioides brasiliensis*and *P. lutzii*, respectively responsible for lacaziosis and paracoccidioidomycosis in humans. Complete identification and phylogenetic classification of the LLD etiological agent still needs to be elucidated, but preliminary phylogenetic analyses have shown a closer relationship of the LLD agent to *Paracoccidioides* spp. than to *L. loboi*. Cases of LLD in South American cetaceans based on photographic identification have been reported; however, to date, only 3 histologically confirmed cases of LLD have been described. We evaluated multiple tissue samples from 4 *Tursiops truncatus* stranded in the states of Santa Catarina (n = 3) and Rio Grande do Sul (n = 1), southern Brazil. Macroscopically, all animals presented lesions consistent with LLD. Hematoxylin-eosin, periodic acid-Schiff, Grocott’s methenamine silver, and Mayer’s mucicarmin stains were used for histological evaluation. Microscopically, numerous refractile yeasts (4-9 µm in diameter) were observed in skin samples (4/4), and for the first time in dolphins, also in a skeletal muscle abscess (1/4). Immunohistochemistry using anti-*P. brasiliensis* glycoprotein gp43 as a primary antibody, which is known to cross-react with *L. loboi* and the LLD agent, was performed and results were positive in all 4 cases. We describe 3 new cases of LLD in cetaceans based on histopathology and immunohistochemistry. This is the first report of LLD in the muscle of cetaceans. http://www.int-res.com/abstracts/dao/v117/n3/p229-235/ Articles available at link above or email: carlosvet.sac@gmail.com Best regards Carlos Sacristán -- *Carlos Sacristán Yagüe* DVM, MSc, PhD Candidate of Department of Pathology - VPT Laboratory of Comparative Wildlife Pathology - LAPCOM School of Veterinary Medicine and Animal Sciences - FMVZ University of São Paulo (Brazil) https://www.linkedin.com/pub/carlos-sacristan-yague/50/266/854
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Carlos Sacristan