New publication: Multimodal assessment of bottlenose dolphin auditory nuclei using 7-Tesla MRI, immunohistochemistry and stereology
Dear colleagues, My co-authors and I are very happy to share our most recent publication on the *Multimodal assessment of bottlenose dolphin auditory nuclei using 7-Tesla MRI, immunohistochemistry and stereology*. You will find the open access publication here: https://doi.org/10.3390/vetsci9120692 Citation: Orekhova, K.; Selmanovic, E.; De Gasperi, R.; Gama Sosa, M.A.; Wicinski, B.; Maloney, B.; Seifert, A.; Alipour, A.; Balchandani, P.; Gerussi, T.; Graïc, J.-M.; Centelleghe, C.; Di Guardo, G.; Mazzariol, S.; Hof, P.R. Multimodal Assessment of Bottlenose Dolphin Auditory Nuclei Using 7-Tesla MRI, Immunohistochemistry and Stereology. *Vet. Sci.* *2022*, *9*, 692. Abstract: The importance of assessing neurochemical processes in the cetacean brain as a tool for monitoring their cognitive health and to indirectly model human neurodegenerative conditions is increasingly evident, although available data are largely semiquantitative. High-resolution MRI for post-mortem brains and stereology allow for quantitative assessments of the cetacean brain. In this study, we scanned two brains of bottlenose dolphins in a 7-Tesla (7T) MR scanner and assessed the connectivity of the inferior colliculi and ventral cochlear nuclei using diffusion tensor imaging (DTI). Serial thick sections were investigated stereologically in one of the dolphins to generate rigorous quantitative estimates of identifiable cell types according to their morphology and expression of molecular markers, yielding reliable cell counts with most coefficients of error <10%. Fibronectin immunoreactivity in the dolphin resembled the pattern in a human chronic traumatic encephalopathy brain, suggesting that neurochemical compensation for insults such as hypoxia may constitute a noxious response in humans, while being physiological in dolphins. These data contribute to a growing body of knowledge on the morphological and neurochemical properties of the dolphin brain and highlight a stereological and neuroimaging workflow that may enable quantitative and translational assessment of pathological processes in the dolphin brain in the future. Please feel free to contact us for any questions you may have, or if you would be interested in sharing your samples for further analyses on cetacean brains. Best wishes, Ksenia Orekhova _____________________________________________________________ Ksenia Orekhova, DVM, Doctoral candidate Dipartimento di Biomedicina Comparata e Alimentazione (BCA) - Università degli Studi di Padova (It) ksenia.orekhova@phd.unipd.it ksenia.orekhova@studenti.unipd.it
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Ksenia Orekhova