New Publication: Age estimation and growth layer patterns in teeth of crabeater seals: A comparison of techniques
Dear MARMAM colleagues, I hope everyone is safe and healthy, My co-authors and I are pleased to share with you of our recent paper which has just been published on Marine Mammal Science "Age estimation and growth layer patterns in teeth of crabeater seals: A comparison of techniques" by Cebuhar J, Negrete J, Botta S. Abstract: Age estimation of marine mammals provides important information about ecological and life-history parameters. Counting growth layer groups (GLGs) in the dentine and cementum of teeth is the most common technique for age estimation in pinnipeds. In this study, we used acid-etched canines (n = 38) and decalcified stained postcanine sections (n = 40) to calibrate readings in mummified crabeater seals (Lobodon carcinophaga). A subsample of this group received a prior cleaning treatment of boiling the teeth with hydrogen peroxide (H2O2). Ages ranged from 0 to 31 years. Dentine from canines and cementum from postcanines showed higher estimated ages (maximum 31 years) than dentine from postcanines (maximum 24 years), mainly in those teeth that did not receive prior treatment. Boiling with H2O2 has shown to affect cementum structure and, thus, results in underestimated ages, but dentine did not seem to be affected. The results presented here showed that ages can be estimated for the crabeater seal regardless of which tooth/tissue or method is used, at least for seals <13 years old. However, nontreated, stained thin sections of postcanine teeth are recommended because more reliable age estimations in older crabeater seals are obtained. Key-words: aging, etched canines, growth layer groups, Lobodon carcinophaga, stained postcanines The article is available via this link: http://dx.doi.org/10.1111/mms.12749 You can also email me if you have any inquires at jucebuhar@gmail.com Thank you for your attention, Kind regards, Julieta Cebuhar
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Julieta Cebuhar